High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition
نویسندگان
چکیده
We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.
منابع مشابه
High Throughput Formation of Sub-million Lipid Membrane Arrays with an Asymmetric Lipid Composition
We present a novel method to form more than 10,000 asymmetric lipid-bilayer membranes at a time on micro-chamber arrays. In our method, the arrayed asymmetric lipid-bilayers, where lipid compositions are different between inner and outer leaflets, are formed by injecting several kinds of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry ...
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